Reprogramming somatic cells into pluripotent em- bryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach forgeneratingpatient-matchednucleartransfer(NT)- ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to pro- duce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identi- fied premature exit from meiosis in human oocytes and suboptimal activation as key factors that are responsible for these outcomes. Optimized SCNT approaches designed to circumvent these limita- tions allowed derivation of human NT-ESCs. When applied to premium quality human oocytes, NT- ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. Gene expression and differ- entiation profiles in human NT-ES
Voir : http://download.cell.com/pdf/PIIS0092867413005710.pdf?intermediate=true
